Whole Transcript Expression Microarray Pro?ling An

full Transcript Expression Micro start out Pro?ling and Sequencing of regulation Colon and Adenocarcinoma natural ribonucleic acid
Stephanie Fulmer-Smentek1, Becky Mullinax2, Eric Lin1, Jennifer Venneri2, Barbara Novak1, Vinayak Kulkarni1 and Anne Bergstrom Lucas1
Genomics R&D, Agilent Technologies, 1Santa Clara CA, and 2La Jolla CA, USA

Introduction
The ability to criterion alternative splicing of exons from RNA that leads to translation of different masterteins is effectual for understanding biological mechanisms and classifying tissue and tumor types. Several methods ar available to interrogate alternative RNA splicing, including exon microarrays, targeted RNA sequencing and full transcriptome RNA sequencing. The SurePrint G3 exon microarray work?ow includes catalog and fashion microarrays, a whole transcript ampli?cation method and tailored abridgment software. Exon-level probes on the microarrays were designed from high-quality content from public databases including RefSeq and Ensembl. Exon array data were analyzed for gene-level and exon-level expression using GeneSpring GX 11.5 software, enabling whole transcript pro?le comparisons within two days. Whole transcriptome RNA sequencing allows for hypothesisneutral discovery of alternative splicing, but at a relatively high cost and low throughput.



In this study, we investigated the correlation of exon microarrays with whole transcriptome RNA sequencing methodologies, demonstrating the high reproducibility and dewy-eyed dynamic range of expression across a compartmentalisation of RNA inputs for the exon microarrays. The whole transcriptome exon microarray work?ow was only used to detect alternative splicing of exons between cancerous and normal cells, resulting in gene and exon expression pro?les consistent with current literature. Our results demonstrate the compatibility and utility of the various methods for measuring rod exon-speci?c expression and alternative splicing.

Microarray LIQA WT Assay Work?ow
Total RNA #1 (Adenocarcinoma or MAQC A) Low...If you want to get a full essay, order it on our website: Orderessay



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